一、《Molecular Cloning 3》
英文版，主要介绍分子克隆的基本实验技术操作，新手如果看不明白此版，可以自己买一本军科院翻译的中文版，感谢lee202525战友提供链接。
Table of Contents
Chapter 1: Plasmids and Their Usefulness in Molecular Cloning
Chapter 2: Bacteriophage and Its Vectors
Chapter 3: Working with Bacteriophage M13 Vectors
Chapter 4: Working with High-Capacity Vectors
Chapter 5: Gel Electrophoresis of DNA and Pulsed-Field Agarose
Chapter 6: Preparation and Analysis of Eukaryotic Genomic DNA
Chapter 7: Extraction, Purification, and Analysis of mRNA from Eukaryotic Cells
Chapter 8: In Vitro Amplification of DNA by the Polymerase Chain Reaction
Chapter 9: Preparation of Radiolabeled DNA and RNA Probes
Chapter 10: Working with Synthetic Oligonucleotide Probes
Chapter 11: Preparation of cDNA Libraries and Gene Identification
Chapter 12: DNA Sequencing
Chapter 13: Mutagenesis
Chapter 14: Screening Expression Libraries
Chapter 15: Expression of Cloned Genes in Escherichia coli
Chapter 16: Introducing Cloned Genes into Cultured Mammalian Cells
Chapter 17: Analysis of Gene Expression in Cultured Mammalian Cells
Chapter 18: Protein Interaction Technologies
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二、Subcloning Notebook
Subcloning is a basic molecular biology procedure used to move inserts from one vector to another. Essentially all subcloning reactions proceed as follows: You release and purify your insert from the parent vector, ligate the insert into a prepared destination vector, transform the ligation reaction into competent bacterial cells, then screen the transformed cells for the insert. The Subcloning Notebook will lead you through every step in this process.
Table of Contents Page
Chapter 1: Classic Subcloning (.pdf, 845kb)
Basic Steps for Subcloning
Subcloning Strategy
Restriction Digestion
Double Enzyme Digests
Partial Restriction Digestion
Creating Blunt Ends
Dephosphorylating Vectors
Ligation
Purifying Vector and Insert
Gel Electrophoresis
DNA Markers
Ordering Information 3
Chapter 2: PCR Subcloning (.pdf, 488kb)
Introduction
T-Vector Systems
Giving Blunt-Ended DNA an A-Tail for T-Vector Subcloning
Subcloning with RE Sites
Subcloning using PCR Primers Containing Restriction Sites
Ordering Information 35
Chapter 3: Transforming Bacteria (.pdf, 366kb)
Properties of E. coli Strains for Subcloning
Ready-to-Use Competent Cells
Determining Transformation Efficiency of Competent Cells
Transforming Ligation Reactions
Media and Solutions 43
Chapter 4: Screening for Recombinants (.pdf, 431kb)
Introduction
Colony PCR
Go Directly to Gel
Screening by Plasmid Minipreps and RE Digests
Plasmid Minipreps
Troubleshooting Subcloning Experiments
Ordering Information 49
Chapter 5: Technical Appendix (.pdf, 274kb)
Restriction Enzyme Activity in 10X Buffers, Reaction Temperature
and Heat Inactivation
Isoschizomers
Compatible Ends
Site-Specific Methylation Sensitivity of Promega Restriction Enzymes
Restriction Enzyme Buffer Composition
Copy Number of Commonly Used Plasmids
Star Activity
Genotypes of Frequently Used Bacterial Strains
Genetic Markers in E. coli
Nucleic Acid Calculations
Formulas for DNA Molar Conversions
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三、丁香园分子克隆参考1.0
为DXY战友自己编写，概括了战友大量的实验体会，相信对新手很有帮助！
主要内容
第一章 质粒DNA的碱裂解法提取与纯化
第二章 基因组DNA的碱裂解法提取与纯化
第三章 真核细胞DNA的制备与定量
第四章 λ噬菌体DNA提取
第五章 核酸电泳
第六章 感受态细胞的制备
第七章 质粒转化
第八章 DNA分子的限制性内切酶消化
第九章 DNA片段回收与纯化
第十章 目的基因的亚克隆
第十一章 PCR产物的克隆
第十二章 DNA序列测定
第十三章 Southern 杂交
第十四章 Northern Blot
第十五章 原位杂交
第十六章 RNA的制备
第十七章 PCR及引物设计
第十八章 RT-PCR
第十九章 实时定量PCR及其它PCR应用
第二十章 EMSA
第二十一章 原核表达及其表达载体
第二十二章 真核表达、真核转染及其表达载体
第二十三章 蛋白SDS电泳
第二十四章 Western
第二十五章 表达蛋白的分离与纯化
第二十六章 表达蛋白的生物学活性的检测
第二十七章 基因治疗与基因治疗载体
第二十八章 基因芯片技术
第二十九章 分子生物学常用软件介绍
第三十章 实验室常用试剂及配制
第三十一章 常用试剂与耗材
文件太大，下载请到核酸版网络硬盘
http://www.dxy.cn/bbs/post/view?bid=64&id=1317517&sty=3&tpg=1&age=0 
四、Cloning Enzymes
Cloning Enzymes, one in the Enzyme Resource Guide series, highlights those enzymes important in nucleic acid cloning procedures. Enzymes that modify nucleic acids provide the foundation for many molecular biology techniques: enzymes that are used to synthesize, degrade, join or remove portions of nucleic acids in a controlled and generally defined manner. Specific features of the in vivo functions of these enzymes have been exploited in vitro to provide many of the protocols currently used in nucleic acid manipulations.This guide mainly is targeted Ligases, Kinases and Phosphatases.
Table of Contents Page
Enzyme Activities Diagram
The Cloning Enzymes
Gene Cloning
Ligases
Kinases and Phosphatases
RecA Protein and AgarACE® Enzyme
Technical Appendix
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五、Nucleic Acid Purification Systems
The isolation and purification of DNA is crucial to many applications in molecular biology and techonogy. Over the past decade, the purification of DNA has evolved from a multi-step process involving organic chemicals to a much simpler process, that is safer and faster. Promega continues to develop new DNA purification products to meet the diverse and changing needs of today's life science researcher. This Nucleic Acid Purification Systems guide presents our many new and innovative products for use in DNA and RNA purification.
Table of Contents Page
Genomic DNA
Plasmid DNA
Fragment DNA
Total RNA
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六、 RNA Analysis Notebook
RNA analysis is the starting point for many molecular biology procedures. The RNA Analysis Notebook provides an introduction to RNA procedures such as purifying RNA, amplifying via RT-PCR, making RNA in vitro and analyzing RNA by microarray. In addition, the RNA Analysis Notebook highlights products for performing these procedures.
Table of Contents
Working with RNA
Purifying RNA and mRNA
Amplifying RNA with RT-PCR
Analyzing RNA with Microarrays
Making RNA in vitro
Silencing RNA in vivo (RNAi)
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七、Restriction Enzymes Resource
Restriction enzymes (REs) that are most useful for molecular biology applications possess two essential attributes: high sequence specificity & precision cutting.
The Restriction Enzymes Resource is an interactive tool, designed to speed your research applications, for identifying REs and RE recognition sites.
Other information are available on NEB online.
Table of Contents
General Information
Applications and Reaction Conditions
Reference Information
Online Searches
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八、PCR Protocols & Reference
PCR is central to molecular biology. Although in principle a simple process, PCR can be difficult to optimize. Furthermore, new variations of the technique appear at an alarming rate. Our guide provides a convenient source of information on this technique, including protocols and troubleshooting tips for both PCR and RT-PCR.
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Table of Contents
1. Introduction to PCR
2. PCR Optimization
3. Example of a PCR Protocol
4. Introduction to RT-PCR
5. RT-PCR Optimization
6. Example of an RT-PCR Protocol
7. Troubleshooting PCR and RT-PCR
8. References
9. Literature & Ordering Information 
九、DNA Analysis Notebook
DNA analysis is the starting point for many molecular biology procedures. The DNA Analysis Notebook provides an introduction to DNA procedures such as purifying genomic DNA, amplifying via PCR, retrieving DNA from PCR and cloning PCR DNA. In addition, the DNA Analysis Notebook features Promega products for performing these procedures.
Contents
Table of Contents
Genomic DNA Purification
Amplifying DNA
PCR Clean-Up
Cloning PCR DNA
DNA Analysis Tools
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Beginning Molecular Biology Laboratory Gudie
新手入门的好教材，非常全面，非常亲切。值得收藏。
http://www.research.umbc.edu/~jwolf/method1.html
内容介绍：
CHAPTER 1: General Laboratory Methods
Safety Procedures
Preparation of Solutions
Disposal of Buffers and Chemicals
Equipment Micropipets
Using a pH meter
Autoclave operating procedures
Operating instructions for spectrophotometer
Working with DNA
Sterile Technique
CHAPTER 2: Instructions for Notebook Keeping
CHAPTER 3: Computer User's Guide
UNIX commands
File Transfer
The World Wide Web
The Wisconsin Package - gcg.
CHAPTER 4: Molecular Biology Methods
M.1: Preparation of genomic DNA from bacteria
M.2: PCR amplification of DNA
M.3: Restriction enzyme digestion of DNA
M.4: Phenol/chloroform extraction of DNA
M.5: Ethanol precipitation of DNA
M.6: Agarose gel electrophoresis
M.7: Transformation of E. coli by electroporation
M.8: Wizard PCR preps DNA purification system
M.9: Alternate method for purifying DNA from agarose gels
M.10: Transfection of mammalian cells using Lipofectamine (LTI)
M.11: Southern blotting
M.12: RT-PCR Protocol
M.13: Preparation of sequencing gels
M.14: Isolation of RNA from mammalian cells using RNAZOL (Teltest)
CHAPTER 5: Tissue Culture Methods
Types of cells grown in culture
Work area and equipment
Preservation and storage
Maintenance
Safety considerations
Tissue culture methods
Determining cell counts
分子克隆molecular cloning3（强烈推荐）
http://mededucation.bjmu.edu.cn/medsite/mc3.htm
编辑：绵子
作者: ggjun
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