Melan-A特T细胞M450-AVT和M280s的排序和剧目的多样性分析
转载请注明来自丁香园
发布日期: 2012-07-25 13:26 文章来源: 丁香园
关键词: 生物专题 四聚体 旷博生物 丁香园 丁香通 点击次数：


点击图片查看全文

M450-AvT and M280s sort of Melan-A speciWc T cell  and analysis of repertoire diversity. a PBMC or TIL from three melanoma patients were stimulated with the Melan-A26–35 A27L peptide for 14 days, sorted with HLA-A*0201/Melan-A-27L multimers made with either M450-AvT or M280s beads and restimulated non speciWcally for 14 days. Percentages of speciWc T cells was assessed by double staining with PE-conjugated tetramer and FITC-conjugated anti-CD8 antibody. As indicated, patient 1 was tested in two independent experiments. b Repertoire diversity of M450 (black bar) and M280 (grey bar) sorted populations was assessed by labeling with 24 antiVβ antibodies


 以下网友留言只代表网友个人观点，不代表网站观点



请输入验证码：