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Binding of MHC/peptide multimers is dependent on the specificity of the TCR. (A) Lymph node cells from 2C and OT-I mice were depleted of B cells and CD41 cells and stained for 1 h at 378C with the indicated PE-conjugated multimers (Mult.). The mean fluorescence intensity (MFI) of 2C cells was 401 when stained with the SIY/Kb multimer versus 4 using the OVA/Kb multimer. MFI for OT-I cells was 5 for the SIY/Kb multimer and 326 with the OVA/ Kb multimer. (B) Staining as in A, except these cells were stained with the anti-CD8a antibody 53.6.7 in addition to the indicated multimer. Numbers represent the percentages of cells in respective quadrants.