| 发布日期: 2011-09-21 15:54 | 文章来源: 欧易生物 | 点击次数： |
Calin, GA; Sevignani, C; Dan Dumitru, C; Hyslop, T; Noch, E; Yendamuri, S; Shimizu, M; Rattan, S; Bullrich, F; Negrini, M; Croce, CM Proc. Natl. Acad. Sci. U. S. A. 2004,101(9)2999-3004 引用次数 904
这篇2004年发表于PNAS的文章阐述了miRNA，特别是肿瘤相关miRNA在染色体上的特殊，即肿瘤相关miRNA经常定位于一些染色体高变区域，可能导致LOH，缺失以及扩增等，从而引起miRNA表达变化或突变、缺失等，影响下游肿瘤相关基因表达，导致肿瘤发生。
A large number of tiny noncoding RNAs have been cloned and named microRNAs(miRs). Recently, we have reported that miR-15a and miR-16a, located at 13q14, are frequently deleted and/or down-regulated in patients with B cell chronic lymphocytic leukemia, a disorder characterized by increased survival. To further investigate the possible involvement of miRs in human cancers on a genome-wide basis, we have mapped 186 miRs and compared their location to the location of previous reported nonrandom genetic alterations. Here, we show that miR genes are frequently located at fragile sites, as well as in minimal regions of loss of heterozygosity, minimal regions of amplification (minimal amplicons), or common breakpoint regions. Overall, 98 of 186 (52.5%) of miR genes are in cancer-associated genomic regions or in fragile sites. Moreover, by Northern blotting, we have shown that several miRs located in deleted regions have low levels of expression in cancer samples. These data provide a catalog of miR genes that may have roles in cancer and argue that the full complement of miRs in a genome may be extensively involved in cancers.
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