| 发布日期: 2011-09-21 16:08 | 文章来源: 欧易生物 | 点击次数： |
Lewis, BP; Burge, CB; Bartel, DP Cell 2005,120(1)15-20 引用次数 2061
这篇发表于2005年的Cell paper截至2011年8月15日引用次数已达2061次。该文章首次提出种子序列的概念，即miRNA中第2-7个核苷酸为与mRNA严格互补的保守的“种子序列”。该文章对miRNA研究产生了重要影响。
【摘要】We predict regulatory targets of vertebrate microRNAs (miRNAs) by identifying mRNAs with conserved complementarity to the seed (nucleotides 2-7) of the miRNA. An overrepresentation of conserved adenosines flanking the seed complementary sites in mRNAs indicates that primary sequence determinants can supplement base pairing to specify miRNA target recognition. In a four-genome analysis of 3' UTRs, approximately 13,000 regulatory relationships were detected above the estimate of false-positive predictions, thereby implicating as miRNA targets more than 5300 human genes, which represented 30% of our gene set. Targeting was also detected in open reading frames. In sum, well over one third of human genes appear to be conserved miRNA targets.
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