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1、Stem Cell Analysis干细胞分析（功能、表型、核酸含量、边群）[London Research Institute]

brief introduction：Stem Cell Analysis ,FACS Laboratory, London Research Institute
干细胞分析，包括以下四方面内容：
Functional Analysis
Phenotyping
Nucleic Acid content
Side Population analysis

2、Production of ES Cell Chimeras by Aggregation wtih Eight-Cell Stage Embryos [Nagy Lab，Mount Sinai Hospital]

brief introduction：Production of ES Cell Chimeras by Aggregation wtih Eight-Cell Stage Embryos
[Nagy Lab，Mount Sinai Hospital]

3、Production of Completely ES Cell-Derived Fetuses by Aggragation with Tetraploid Embryos[Nagy Lab，Mount Sinai Hospital]

brief introduction：Production of Completely ES Cell-Derived Fetuses by Aggragation with Tetraploid Embryos
Nagy Lab，Mount Sinai Hospital

4、Picking ES Cell Colonies and Transferring Them to 24-Well Culture Dishes

brief introduction：Picking ES Cell Colonies and Transferring Them to 24-Well Culture Dishes
From the Laboratory of Dr. Allan Bradley Baylor College of Medicine, Houston, Texas

5、Preparation of Feeder Layers And SNL Stocks [Baylor College of Medicine]

brief introduction：Preparation of Feeder Layers And SNL Stocks ,
Allan Bradley's Lab, Baylor College of Medicine, Houston, Texas

6、Preparing 48-Well Plate Feeders[Baylor College of Medicine]

brief introduction：Preparing 48-Well Plate Feeders
From the Laboratory of Dr. Allan Bradley ,Baylor College of Medicine, Houston, Texas

7、Freezing Embryonic Stem (ES) Cell Clones in 96-Well Plates冻存96空板中的胚胎干细胞克隆[Baylor College of Medicine]

brief introduction：Freezing Embryonic Stem (ES) Cell Clones in 96-Well Plates
the Laboratory of Dr. Allan Bradley ,Baylor College of Medicine, Houston, Texas

8、In Vitro Differentiate ES cells into glial cells and neurons 胚胎干细胞体外分化为神经元和神经胶质细胞(Nagy Lab，Mount Sinai Hospital)

brief introduction： Differentiate ES cells into glial cells and neurons ,Nagy Lab，Mount Sinai Hospital
胚胎干细胞体外分化为神经元和神经胶质细胞

9、In Vitro Differentiation of ES Cells into Cystic Embryoid Bodies 胚胎干细胞体外分化为囊胚体(Nagy Lab，Mount Sinai Hospital)

brief introduction：In Vitro Differentiation of ES Cells into Cystic Embryoid Bodies ,Nagy Lab，Mount Sinai Hospital
胚胎干细胞体外分化为囊胚体

10、In Vitro Differentiation of ES Cells into Cardiac Muscle胚胎干细胞体外分化成心肌细胞 (Nagy Lab，Mount Sinai Hospital )

brief introduction：In Vitro Differentiation of ES Cells into Cardiac Muscle
Nagy Lab，Mount Sinai Hospital

11、Isolation of Primary Fibroblasts from Mouse Embryos 从小鼠胚胎分离纤维原细胞Baylor College of Medicine

brief introduction：Isolation of Primary Fibroblasts from Mouse Embryos ,
Allan Bradley's Lab, Baylor College of Medicine, Houston, Texas

12、ES Cell Subcloning Protocol 胚胎干细胞亚克隆(NIH Stem Cell Unit)

brief introduction：ES Cell Subcloning Protocol, NIH ，Stem Cell Unit

13、ES and TS cell freezing/thawing胚胎干细胞冻存复苏

brief introduction：ES and TS cell freezing/thawing, Nagy Lab,Samuel Lunenfeld Research Institute, Room 881 ,Mount Sinai Hospital
该实验室专著于小鼠遗传学以及其跟人类疾病关系

14、ES Cell Culture and Manipulation (The Cancer Center at Washington University)

brief introduction：ES Cell Culture and Manipulation (The Cancer Center at Washington University)

15、ES / MEF cell culture and electroporation of targeting construct 胚胎干细胞培养及电穿孔

brief introduction：This website is an online resource for those interested in murine transgenesis. Within this site you will find information pertaining to research and techniques employed in the field of transgenics, including both DNA microinjection and embryonic stem cell injection.

16、Electroporation of ES Cell 胚胎干细胞电穿孔[Mount Sinai Hospital]

brief introduction：Electroporation of ES Cell ,Nagy Lab,Samuel Lunenfeld Research Institute, Room 881 ,Mount Sinai Hospital
胚胎干细胞电穿孔，该实验室专著于小鼠遗传学以及其跟人类疾病关系

17、Culture of Human Embryonic Stem Cells (hESC)人胚胎干细胞培养[NIH Stem Cell Unit]

brief introduction：Culture of Human Embryonic Stem Cells (hESC),NIH Stem Cell Unit
人胚胎干细胞培养，来自NIH Stem Cell Unit的材料，该网址下还有许多NIH关于干细胞的资料。

18、Analysis of ES Cell Clones by Mini-Southern 分析胚胎干细胞克隆（Baylor College of Medicine）

brief introduction：Analysis of ES Cell Clones by Mini-Southern ,Allan Bradley's Lab, Baylor College of Medicine, Houston, Texas,Mini-southern法
分析胚胎干细胞克隆

19、General ES Cell Protocols胚胎干细胞基本操作（Baylor College of Medicine）

brief introduction：EMBRYONIC STEM CELLS PROTOCOL,Allan Bradley's Lab, Baylor College of Medicine, Houston, Texas

20、Stem Cell Protocols 很全的干细胞操作[University of Wisconsin]

brief introduction：Stem Cell Protocols Thomson Lab, University of Wisconsin
内容很全的干细胞基本操作;主要包括以下内容：
Media and reagents
Thawing ES Cells
Splitting ES Cells on MEF's (stem cell photos)
Freezing ES Cells
Matrigel Aliquoting and Plating
Splitting ES Cells on Matrigel
Embryonic Bodies


edited by sdimmuno