bisulfite sequencing PCR
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发布日期: 2012-09-20 21:02 | 文章来源: 丁香园 |
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Bisulfite sequencing PCR (BSP): DNA is first denatured to create single-stranded DNA and then modified with sodium bisulfite followed by PCR amplification using primers that are specific for the modified DNA but do not contain any CpG sites in their sequence. The resulting PCR product is sequenced directly or after cloning
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