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Normalized melt data of the MS-HRM assays for CD14_1 (A and C) and CD14_2 (B and D). (A and B) standard curves in colored lines are composed of mixed Epitect (Qiagen) unmethylated and methylated bisulfite-converted human control DNA (methylation level: 0% red, 10% orange, 25% green, 50% blue, and 100% pink). (C and D) standard curves in black lines and the 36 placental samples from set A in colored lines according to MS-HRM clustering (C: cluster: 1 light blue, 2 dark red. D: cluster:1 brown, 2 gray, 3 turquoise, and 4 purple).

图片出处：DNA methylation levels within the CD14 promoter region are lower in placentas of mothers living on a farm.Allergy. 2012 Jul.