精彩细胞图片（八）
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PC12 细胞未分化与分化后图片
培养基：Ham's F12K medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 82.5%; horse serum, 15%; fetal bovine serum, 2.5%
传代：Remove cells from substrate by pipetting over the cell sheet with fresh medium, by striking flask or by scraping. Aspirate cells in fresh medium with a small bore pipette to break up clusters. Add fresh culture medium, aspirate and dispense into new culture flasks. 慎用胰酶，因为pc12是半贴壁细胞，吹打后继可传代。pc12生长时有形成cluster的趋势。
【标准细胞】Bovine Brain Microvascular Endothelial Cells (BBMVEC) 脑微血管内皮细胞
Bovine Brain Microvascular Endothelial Cells (BBMVEC) are isolated from microvessels of bovine brain. The cells are cryopreserved at second passage. BBMVEC can be cultured and propagated for at least 12 population doublings. Brain Microvascular Endothelial Cells form the basis of the blood-brain barrier, and have been used as a model for research on bacterial meningitis[1],2,3. Hypoxia-ischemia studies showed that BBMVEC cell death may exacerbate ischemic brain injury4. BBMVEC have also been used to show oxyhemoglobin induces apoptosis in vascular endothelial cells5. Co-culture with Atrocytes generate an in vitro model that closely mimics the in vivo situation for evaluating drug transport6.
【标准细胞】Rat Brain Microvascular Endothelial Cells(RBMVEC)
Rat Brain Microvascular Endothelial Cells (RBMVEC) are derived from the brain of adult Sprague Dawley rat. The cells are cryopreserved at second passage. RBMVEC can be cultured and propagated 16 population doublings. HIV-1 Tat protein alters the tight junction protein expression and distribution in cultured brain endothelial cells. It is believed such alteration lead to disturbances of the blood-brain barrier (BB integrity and contributes to HIV trafficking in to the brain1. Endothelial cell-derived monocyte-chemoattractant protein-1 (MCP-1) plays a key role in leukocyte recruitment across the blood-brain and blood-retinal barriers in vivo2 . Three-dimensional model indicates that dipyridamole can improve BBB Function3. Mainteance of BBB function during and following normoxic-cormoglycemic flow cessation appears to depend on intact nitric oxide signaling and IL-6 release4. Expression of metabotropic glutamate receptors may modulate synaptic transmission and determine microvascular function and dysfunction5. VEGF mRNA and VEGF receptor F1K-1/KDR are both higher in neonatal than in adult microvessels suggesting that microvascular cell turnover continues in the adult brain6. Hypoxia increases the aparacellualr flux across the cell monolayer via the release of VEGF, which in turn leads to the dislocalization, decreased expression and enhanced phosphorylation of Zonula occludens-1(ZO-1)7.
人血管平滑肌细胞（原代）
1.细胞种类：人大隐静脉平滑肌细胞；
2.培养的天数：组织块接种2周后见细胞爬出，继续培养10天；
3.放大倍速：40倍；
4.培养基种类：DMEM+20%胎牛血清；
5.细胞状态与特征简述：贴壁生长，呈长梭形。
培养基：Ham's F12K medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 82.5%; horse serum, 15%; fetal bovine serum, 2.5%
传代：Remove cells from substrate by pipetting over the cell sheet with fresh medium, by striking flask or by scraping. Aspirate cells in fresh medium with a small bore pipette to break up clusters. Add fresh culture medium, aspirate and dispense into new culture flasks. 慎用胰酶，因为pc12是半贴壁细胞，吹打后继可传代。pc12生长时有形成cluster的趋势。
【标准细胞】Bovine Brain Microvascular Endothelial Cells (BBMVEC) 脑微血管内皮细胞
Bovine Brain Microvascular Endothelial Cells (BBMVEC) are isolated from microvessels of bovine brain. The cells are cryopreserved at second passage. BBMVEC can be cultured and propagated for at least 12 population doublings. Brain Microvascular Endothelial Cells form the basis of the blood-brain barrier, and have been used as a model for research on bacterial meningitis[1],2,3. Hypoxia-ischemia studies showed that BBMVEC cell death may exacerbate ischemic brain injury4. BBMVEC have also been used to show oxyhemoglobin induces apoptosis in vascular endothelial cells5. Co-culture with Atrocytes generate an in vitro model that closely mimics the in vivo situation for evaluating drug transport6.
【标准细胞】Rat Brain Microvascular Endothelial Cells(RBMVEC)
Rat Brain Microvascular Endothelial Cells (RBMVEC) are derived from the brain of adult Sprague Dawley rat. The cells are cryopreserved at second passage. RBMVEC can be cultured and propagated 16 population doublings. HIV-1 Tat protein alters the tight junction protein expression and distribution in cultured brain endothelial cells. It is believed such alteration lead to disturbances of the blood-brain barrier (BB integrity and contributes to HIV trafficking in to the brain1. Endothelial cell-derived monocyte-chemoattractant protein-1 (MCP-1) plays a key role in leukocyte recruitment across the blood-brain and blood-retinal barriers in vivo2 . Three-dimensional model indicates that dipyridamole can improve BBB Function3. Mainteance of BBB function during and following normoxic-cormoglycemic flow cessation appears to depend on intact nitric oxide signaling and IL-6 release4. Expression of metabotropic glutamate receptors may modulate synaptic transmission and determine microvascular function and dysfunction5. VEGF mRNA and VEGF receptor F1K-1/KDR are both higher in neonatal than in adult microvessels suggesting that microvascular cell turnover continues in the adult brain6. Hypoxia increases the aparacellualr flux across the cell monolayer via the release of VEGF, which in turn leads to the dislocalization, decreased expression and enhanced phosphorylation of Zonula occludens-1(ZO-1)7.
人血管平滑肌细胞（原代）
1.细胞种类：人大隐静脉平滑肌细胞；
2.培养的天数：组织块接种2周后见细胞爬出，继续培养10天；
3.放大倍速：40倍；
4.培养基种类：DMEM+20%胎牛血清；
5.细胞状态与特征简述：贴壁生长，呈长梭形。
作者: 丁香园集体创作
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