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    利用PHOTOSHOP将两种荧光图象重叠，从而达到激光共聚焦效果，可以节省不少

    操作步骤如下：

    Photoshop Procedure to Colorize Immuno Images
    Open Photoshop
    Open both images (Cy3-red and Hoechest33258-blue)

    1. Choose one image
    Image—Mode—RGB Color
    Image—Adjust—Brightness/Contrast.
    Then Save As:

    2. Choose other image
    Image—Mode—RGB Color
    Image—Adjust—Brightness/Contrast.
    Then Save As:

    3. Select one of the images—(say the Hoechest33258-blue)
    Go to Channels
    Click on Red—Go to Image—Adjust—Brightness/Contrast
    Move both sliders to zero (or -100)
    Click on Green—Go to Image—Adjust—Brightness/Contrast
    Move both sliders to zero (or -100)
    Save

    4. Select the other image (Cy3-red)
    Go to Channels
    Click on Green—Go to Image—Adjust—Brightness/Contrast
    Move both sliders to zero (or -100)
    Click on Blue—Go to Image—Adjust—Brightness/Contrast
    Move both sliders to zero (or -100)
    Save

    5. Select one of the images
    Go to Layers
    Double click on Background—Rename: Cy3-red
    Save

    6. Select the other image
     Go to Layers
    Double click on Background—Rename: Hoechest33258-blue
    Save

    7. Select All
    Copy

    8. Go to the other image
    Paste
    Change from “Normal” to “Screen” on Layers pallette.
    Save As:

编辑：qingqingruochen