神经干细胞
Neural stem cells (NSCs) are functionally defined by their capacity to self renew and their ability to differentiate into multiple specialized progenitor cells, which can commit to further maturation along specific lineages including astrocytes, neurons, and oligodendrocytes. Ex vivo expanded neural stem cells serve as excellent tools for researchers studying neural development and neurological disorders.
R&D Systems offers media and NSC expansion kits and functional identification kits to direct stem cells along neuronal lineages.
Recent Reference using Neural Stem Cell products from R&D Systems
* Sold under license from StemCells California, Inc. U.S. Patent Nos. 5,750,376; 5,851,832; 5,980,885; 5,968,829; 5,981,165; 6,071,889; 6,093,531; 6,103,530; 6,165,783; 6,238,922.
Serum-Free Medium & Neural Stem Cell Expansion Kits
StemXVivo™ Serum-Free NSC Base Media is a serum-free formulation for the expansion and differentiation of neural stem and progenitor cells. All the components have been selected and optimized for culturing human, mouse, or rat cells. This media must be supplemented with cytokines/growth factors for the desired cell culture application. The cytokine/growth factor combinations used depends upon the experimental design of each researcher. The Neural Stem Cell (NSC) Expansion Kits contain specially formulated reagents to grow NSCs in vitro as monolayer cultures on an adhesive substrate or as free-floating“neurospheres.” Cells grown using Neural Stem Cell Expansion Kits are Nestin-positive and proliferate in culture, while retaining the potential to differentiate into neurons, oligodendrocytes, and astrocytes.
Please see pages 16-19 for a protocol using StemXVivo Serum-Free NSC Base Media for the expansion of rat cortical stem cells.
Neural Differentiation Kits
Kits are available for the serum-free differentiation of embryonic stem (ES) cells into dopaminergic neurons or oligodendrocytes. Each kit contains specially formulated media supplements and a growth factor panel designed to direct the differentiation to the specific neural lineage. These kits contain sufficient reagents for the induction of approximately 3 x 107 ES cells.
Neural Stem Cell Functional Identification Kits
Functional Identification kits are designed for in vitro identification of human or mouse/rat neural stem cells based on their ability to differentiate into multiple neural lineages. These kits contain specially formulated media supplements and a panel of antibodies for the differentiation and identification of neural precursors, astrocytes, neurons, and oligodendrocytes.
Neural Stem Cell Functional Identification Kits
Functional Identification kits are designed for in vitro identification of human or mouse/rat neural stem cells based on their ability to differentiate into multiple neural lineages. These kits contain specially formulated media supplements and a panel of antibodies for the differentiation and identification of neural precursors, astrocytes, neurons, and oligodendrocytes.
Expansion, Differentiation, and Identification of Neural Cell Populations using the Mouse/Rat NSC Functional Identification Kit. Nestin detected in rat cortical stem cells (Catalog # NSC001) with anti-rat Nestin (Figure 1; red). Three distinct cell types in differentiated neural stem cells were identified using the Mouse/Rat Neural Stem Cell Functional Identification Kit (Catalog # SC013). Neurons were detected using anti-neuron-specific b-III Tubulin (Figure 2A; red); astrocytes using anti-human GFAP (Figure 2A; green); and oligodendrocytes using anti-O4 (Figure 2B; red). Primary antibodies are included in the Neural Stem Cell Functional Identification Kit.
Primary Rat Cortical Stem Cells
R&D Systems offers ready-to-use primary cortical stem cells isolated from E14.5 Sprague-Dawley rats. Every lot is validated for high levels of Nestin expression and the capacity for multi-lineage differentiation (astrocytes, neurons, and oligodendrocytes). Depending on your research needs, Rat Cortical Stem Cells can be optimally expanded as monolayers or neurospheres.
Please see pages 16-19 for a protocol using StemXVivo Serum-Free NSC Base Media for the expansion of rat cortical stem cells.
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