默克密理博生物科学家Philip Lee
Philip Lee, Ph.D., Merck-Millipore/CellASIC, Hayward, CA, USA
Work Experience
Philip Lee is a Research Fellow at Merck-Millipore, and previously Director of R&D and CEO of CellASIC Corp prior to the company’s acquisition by Merck-Millipore in 2012. Philip co-founded CellASIC in 2005 and helped pioneer technology development on microfluidic cell culture. Philip’s current research focuses on the interface of microfluidic engineering and cell biology to deliver more physiologically relevant and predictive tools for biological research.
Philip received his Ph.D. from the UC Berkeley/UC San Francisco Joint Bioengineering Group under Professor Luke Lee, working on microfluidic technologies to address applications in cellular systems biology. Prior to Berkeley, Philip obtained undergraduate degrees in Chemical Engineering and Biology at the Massachusetts Institute of Technology. In the past 10 years, Philip has authored 19 scientific papers, named inventor on 8 patent applications, and presented at over 20 multi-national scientific meetings. His awards include an R&D100 invention for 2010, the Laboratory Automation New Product Award in 2011, R&D Magazine Micro/Nano 25 Top Technologies of 2006, the NSF Graduate Research Fellowship, and numerous innovation and entrepreneurship awards.
Presentation Topic
Using Microfluidic Engineering to Enable Physiologically Predictive Cell Analysis
Presentation Brief
Understanding and predicting cell phenotype is central to developing improved therapeutics and diagnostics for biomedicine. While a great deal of information is known about molecular mechanisms and pathways, there is currently insufficient knowledge on how this translates to cellular phenotype, and ultimately disease states. The reductionist approach of cell biology has given way to a more complex network of interacting systems within single cells. Traditional static 2D culture methods are insufficient to provide relevant data for this type of experimentation. The advance of microfabrication and microfluidic technologies for cell culture applications enables an unprecedented level of control over the live cell environment. The key benefits of microfluidic cell culture are to 1) create a more biologically relevant in vitro environment to predict in vivo activity, and 2) provide a precise and standardized format that is accessible to biologists. In our work, we have investigated the design of microfluidic cell culture chambers that recreate the mass transport environment of tissues, elicit cell responses to dynamic solution changes, model host-pathogen interactions, and enables long term perfusion culture in a three dimensional extracellular matrix. These microfluidic design features were engineered into a standard format for ease of liquid handling and live cell imaging. A control system was also developed to automate the control of flow, temperature, and gas environment of the cells during experiment. This configuration allows the operation of numerous application specific microfluidic plates, including designs for solution switching, spatial gradients, 3D culture, yeast/bacteria single cell analysis, and a liver perfusion model. Our future work focuses on refining the physiologic models and expanding the set of application specific designs.
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