Directed differentiation of human ES cells and iPS cells into pancreatic insulin-producing cells
Hongkui Deng
Laboratory of Stem Cell and Generative Biology, College of Life Sciences, Peking University, Beijing 100871, China.
Human pluripotent stem cells represent a potentially unlimited source of functional pancreatic endocrine lineage cells. We established a new highly efficient approach to induce human embryonic stem （ES） cells and induced pluripotent stem （iPS） cells to differentiate into mature insulin-producing cells in a chemical-defined culture system. The differentiated cells obtained by this approach comprised nearly 25% insulin-positive cells as assayed by flow cytometry analysis, which released insulin/C-peptide in response to glucose stimuli in a manner comparable to that of adult human islets. Most of these insulin-producing cells co-expressed mature beta cell-specific markers such as NKX6-1 and PDX1, indicating a similar gene expression pattern to adult islet beta cells in vivo. This work provides a new model to study the mechanism of human pancreatic specialization and maturation in vitro, and enhances the possibility of utilizing patient-specific iPS cells for the treatment of diabetes.
编辑: ludongcn 作者:Hongkui Deng
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