Melan-A特T细胞M450-AVT和M280s的排序和剧目的多样性分析
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M450-AvT and M280s sort of Melan-A speciWc T cell and analysis of repertoire diversity. a PBMC or TIL from three melanoma patients were stimulated with the Melan-A26–35 A27L peptide for 14 days, sorted with HLA-A*0201/Melan-A-27L multimers made with either M450-AvT or M280s beads and restimulated non speciWcally for 14 days. Percentages of speciWc T cells was assessed by double staining with PE-conjugated tetramer and FITC-conjugated anti-CD8 antibody. As indicated, patient 1 was tested in two independent experiments. b Repertoire diversity of M450 (black bar) and M280 (grey bar) sorted populations was assessed by labeling with 24 antiVβ antibodies
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