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人类CD8 T细胞被A2Kb四聚体非特异性染色

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发布日期:2012-07-25 13:41 文章来源:丁香园
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关键词: 生物专题 四聚体 旷博生物 丁香园 丁香通   点击次数:



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Nonspecific staining of human CD8+ T cells by A2Kb tetramers. A, SPR equilibrium measurement of soluble human NY-ESO-1157–165 specific TCR binding to immobilized A2 monomers (solid line), A2Kb monomers (dashed line) loaded with cognate peptide and blank control (gray line). B, Binding affinity of human CD8 to A2Kb (dashed line) and A2 (solid line) monomers loaded with the NY-ESO-1157–165 peptide. Kd was measured at room temperature and calculated by Scatchard analysis. C, Binding of A2Kb, A2, and 226/227AK A2Kb tetramers loaded with the NY-ESO-1157–165 peptide to PBL of an A2-positive healthy donor.

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