长链非编码RNA参与调节精子成熟
近日，国际著名杂志PLoS ONE刊登了一篇“Identification and Characterization of a Novel Non-Coding RNA Involved in Sperm Maturation,”文章，文章中作者阐述了关于长链非编码RNA以小RNA前体分子形式参与调节精子成熟的相关工作。
小RNA分子近年来成为研究基因表达调控的热门领域，但其在精子附睾成熟过程中的作用的研究报道却还很少。张永莲组倪敏洁博士等利用大鼠附睾 cDNA 文库筛选克隆到一个附睾特异的新长链非编码RNA分子(HongrES2)。该RNA分子全长1.6kb，有两个exon，并且两个exon来源于两个不同的染色体；具有类mRNA分子的5'端帽子和3'端polyA结构，但却没有开放读码框。其3'端序列和另一个附睾特异编码基因，羧基酯酶ces7的3'端序列完全同源，并能够在细胞水平下调CES7的蛋白表达。
进一步的研究表明，HongrES2能够生成一个23bp的小RNA分子mil-Hongres2(microRNA like HongrES2)，而体内外实验都证明HongrES2对CES7的调节作用即为mil-hongres2对ces7 的直接靶向作用所致。另外该小RNA分子的生成量在正常生理水平很低，受到附睾炎症刺激后短时间内激增，表明其从前体到成熟体的过程受到严格调控；同时观察到如果整体过表达其小分子成熟体，大鼠精子的运动和获能等精子附睾成熟过程均受到影响。这些初期研究结果提示HongrES2以小分子调节RNA(small modulatory RNA，smRNA)的前体形式稳定存在于大鼠附睾组织中，参与维持附睾精子成熟所需特定的微环境，而其是否会在附睾炎中发挥保卫基因的作用还有待于后续的研究证实（来自生物谷）。
原文摘要
Identification and Characterization of a Novel Non-Coding RNA Involved in Sperm Maturation
Min-Jie Ni1, Zhi-Hong Hu1, Qiang Liu1, Mo-Fang Liu2, Min-hua Lu2, Jin-Song Zhang1, Li Zhang1, Yong-Lian Zhang1,3*
The microRNA miR393 has been shown to play a role in plant development and in the stress response by targeting mRNAs that code for the auxin receptors in Arabidopsis. In this study, we verified that two rice auxin receptor gene homologs (OsTIR1 and OsAFB2) could be targeted by OsmiR393 (Os for Oryza sativa). Two new phenotype <http://www.biodic.cn/search.asp?txtitle=phenotype>s (increased tillers and early flowering) and two previously observed phenotype <http://www.biodic.cn/search.asp?txtitle=phenotype>s (reduced tolerance to salt and drought and hyposensitivity to auxin) were observed in the OsmiR393-overexpressing rice plants. The OsmiR393-overexpressing rice demonstrated hyposensitivity to synthetic auxin-analog treatments. These data indicated that the phenotypes of OsmiR393-overexpressing rice may be caused through hyposensitivity to the auxin signal by reduced expression of two auxin receptor genes (OsTIR1 and OsAFB2). The expression of an auxin transporter (OsAUX1) and a tillering inhibitor (OsTB1) were downregulated by overexpression of OsmiR393, which suggested that a gene chain from OsmiR393 to rice tillering may be from OsTIR1 and OsAFB2 to OsAUX1, which affected the transportation of auxin, then to OsTB1, which finally controlled tillering. The positive phenotypes (increased tillers and early flowering) and negative phenotypes (reduced tolerance to salt and hyposensitivity to auxin) of OsmiR393-overexpressing rice present a dilemma for molecular breeding.
编辑: gaowei2010
以下网友留言只代表网友个人观点，不代表网站观点
