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转基因小鼠的实验设计
转基因小鼠在连续移植策略中的应用
FIG. Sequential transplantation strategy.
Donor testis cells of adult ROSA26 transgenic mice were transplanted into recipient testes in two groups of mice. In the control group, donor cells were transplanted once on Day 0 and analyzed 2 mo later to count the number of colonies of spermatogenesis arising from donor SSCs. In the experimental group, donor cells were transplanted into the primary recipient testes on Day 0, and at various intervals after primary transplantation these cells were retransplanted into the secondary recipient testes, which were analyzed 2 mo later. The recolonization rate was determined as a ratio of colony numbers in the experimental vs. control groups, and the lowest rate was considered the homing efficiency.
原文链接：
Homing Efficiency and Proliferation Kinetics of Male Germ Line Stem Cells Following Transplantation in Mice
编辑: jurgen 作者:克林斯曼
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