丁香园 | 丁香通 | 人才 | 会议 | 药学 | 博客    点击次数:

Cloning Eight Years After Dolly

转载请注明来自丁香园
发布日期:2009-02-17 17:27 文章来源:丁香园
关键词: 克隆 多莉 转基因 Dolly   点击次数:
分享到: 收藏夹 新浪微博 腾讯微博 开心网 豆瓣社区 人人网


作者:Campbell, KHS; Alberio, R; Choi, I, et al.
来源出版物:REPRODUCTION IN DOMESTIC ANIMALS   卷: 40   期: 4   页: 256-268   出版年: 2005
被引频次: 20

It is now 8 years since the birth of Dolly, the first animal produced by nuclear transfer using a donor cell
population established from an adult animal. During this time, the technique of nuclear transfer has
been successfully applied to a range of mammalian species for the production of offspring using a
plethora of donor cell types derived from both foetal and adult tissues.

In addition, when coupled with genetic manipulation of the donor cells, transgenic offspring have been
produced with a range of genetic modifications including gene knockouts and gene knockings. Despite
the apparent successes of the technology, the efficiency of development to live offspring has remained
low and developmental abnormalities still occur. The objectives of this paper are to review some of the
successes and failures of the nuclear transfer procedure since the production of Dolly.

In particular, we will review the major steps in the procedure and discuss studies from our laboratory
and others which have modified the procedure in ways which may impact on development.


体细胞克隆的里程碑事件

全文请看“丁香园讨论”

请点这里参加丁香园论坛讨论 >>

编辑: jurgen    作者:丁香园通讯员

以下网友留言只代表网友个人观点,不代表网站观点