Cloning Eight Years After Dolly
作者：Campbell, KHS; Alberio, R; Choi, I, et al.
来源出版物：REPRODUCTION IN DOMESTIC ANIMALS 卷： 40 期： 4 页： 256-268 出版年： 2005
被引频次： 20
It is now 8 years since the birth of Dolly, the first animal produced by nuclear transfer using a donor cell
population established from an adult animal. During this time, the technique of nuclear transfer has
been successfully applied to a range of mammalian species for the production of offspring using a
plethora of donor cell types derived from both foetal and adult tissues.
In addition, when coupled with genetic manipulation of the donor cells, transgenic offspring have been
produced with a range of genetic modifications including gene knockouts and gene knockings. Despite
the apparent successes of the technology, the efficiency of development to live offspring has remained
low and developmental abnormalities still occur. The objectives of this paper are to review some of the
successes and failures of the nuclear transfer procedure since the production of Dolly.
In particular, we will review the major steps in the procedure and discuss studies from our laboratory
and others which have modified the procedure in ways which may impact on development.
体细胞克隆的里程碑事件
来源出版物：REPRODUCTION IN DOMESTIC ANIMALS 卷： 40 期： 4 页： 256-268 出版年： 2005
被引频次： 20
It is now 8 years since the birth of Dolly, the first animal produced by nuclear transfer using a donor cell
population established from an adult animal. During this time, the technique of nuclear transfer has
been successfully applied to a range of mammalian species for the production of offspring using a
plethora of donor cell types derived from both foetal and adult tissues.
In addition, when coupled with genetic manipulation of the donor cells, transgenic offspring have been
produced with a range of genetic modifications including gene knockouts and gene knockings. Despite
the apparent successes of the technology, the efficiency of development to live offspring has remained
low and developmental abnormalities still occur. The objectives of this paper are to review some of the
successes and failures of the nuclear transfer procedure since the production of Dolly.
In particular, we will review the major steps in the procedure and discuss studies from our laboratory
and others which have modified the procedure in ways which may impact on development.
体细胞克隆的里程碑事件
全文请看“丁香园讨论”
编辑: jurgen 作者:丁香园通讯员
以下网友留言只代表网友个人观点，不代表网站观点
