Transgenic animal bioreactors
作者：Louis Marie Houdebine
单位：Unite de Biologie du D´eveloppement et Biotechnologie, Institut National de la
Recherche Agronomique, 78352 Jouy-en-Josas Cedex, France, T´el.: 33 1 34 65 25 40;
Fax: 33 1 34 65 22 41; E-mail: houdebine@biotec.jouy.inra.fr
期刊：Transgenic Research 9: 305–320, 2000.
Abstract
The production of recombinant proteins is one of the major successes of biotechnology.
Animal cells are required to synthesize proteins with the appropriate post-translational
modifications. Transgenic animals are being used for this purpose.
Milk, egg white, blood, urine, seminal plasma and silk worm cocoon from transgenic
animals are candidates to be the source of recombinant proteins at an industrial scale.
Although the first recombinant protein produced by transgenic animals is expected to be
in the market in 2000, a certain number of technical problems remain to be solved before
the various systems are optimized. Although the generation of transgenic farm animals
has become recently easier mainly with the technique of animal cloning using transfected
somatic cells as nuclear donor, this point remains a limitation as far as cost is concerned.
Numerous experiments carried out for the last 15 years have shown that the expression
of the transgene is predictable only to a limited extent.
This is clearly due to the fact that the expression vectors are not constructed in an
appropriate manner.
Key words
Recombinant proteins, transgenic animals, milk
编辑: jurgen 作者:Louis Marie Houdebine
以下网友留言只代表网友个人观点，不代表网站观点
