A Fluorescent Ligand-Binding Alternative Using Tag-lite® Technology

转载请注明来自丁香园
发布日期：2011-10-18 15:30 文章来源：Cisbio
分享到： 收藏夹 新浪微博 腾讯微博 开心网 豆瓣社区 人人网
关键词： 丁香园 生物专题 htrf Tag-lite® Technology 　 点击次数：

By: Zwier JM, Roux T, Cottet M, Durroux T, Douzon S, Bdioui S, Gregor N, Bourrier E, Oueslati N, Nicolas L, Tinel N, Boisseau C, Yverneau P, Charrier-Savournin F, Fink M, Trinquet E.

IGF, Institut National de la Sante et de la Recherche Medicale (Montpellier, France), Cisbio Bioassays (France)

Abstract: G-protein-coupled receptors (GPCRs) are crucial cell surface receptors that transmit signals from a wide range of extracellular ligands. Indeed, 40% to 50% of all marketed drugs are thought to modulate GPCR activity, making them the major class of targets in the drug discovery process. Binding assays are widely used to identify high-affinity, selective, and potent GPCR drugs. In this field, the use of radiolabeled ligands has remained so far the gold-standard method. Here the authors report a less hazardous alternative for high-throughput screening (HTS) applications by the setup of a nonradioactive fluorescence-based technology named Tag-lite®. Selective binding of various fluorescent ligands, either peptidic or not, covering a large panel of GPCRs from different classes is illustrated, particularly for chemokine (CXCR4), opioid (d, µ,), and cholecystokinin (CCK1 and CCK2) receptors. Affinity constants of well-known pharmacological agents of numerous GPCRs are in line with values published in the literature. The authors clearly demonstrate that the Tag-lite binding assay format can be successfully and reproducibly applied by using different cellular materials such as transient or stable recombinant cells lines expressing SNAP-tagged GPCR. Such fluorescent-based binding assays can be performed with adherent cells or cells in suspension, in 96- or 384-well plates. Altogether, this new technology offers great advantages in terms of flexibility, rapidity, and user-friendliness; allows easy miniaturization; and makes it completely suitable for HTS applications. (Journal of Biomolecular Screening Sept 2010)

编辑: cq

以下网友留言只代表网友个人观点，不代表网站观点

昵称：游客 请登录或注册后，发表留言

验证码：