HTRF术语详解 详细 >>
FRET：指荧光共振能量转移，在供体和受体相互靠得很近时，光子能从一个受激发的荧光团（供体）转移到另一个荧光团（受体），并使后者发出荧光。
HTRF：均相时间分辨荧光，Homogeneous Time-Resolved Fluorescence ）是用来检测纯液相体系中待测物的一种常用方法。
背景（Background）：指在没有加入compound时检测到的信号值。
荧光受体（acceptor）：指在荧光团受到激光激发时，能发出特定波长的发射光的基团。HTRF®的能量受体均可为XL665和d2。
资料下载 更多 >>
- New HTRF cellular platform for cell surface receptors' study and screening
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- HTRF GPCR Solutions:Gi,Gs,Gq receptor screening using a single technology
- HTRF Compatible Readers:Unleash your lab's capacity to read HTRF technology
- Forget Elisa use HTRF biomarkers
In vitro modulation of LPS/calcimycin induced inflammatory and allergic mediators by pure compounds of Andrographis paniculata (King of bitters) extract
By: Chandrasekaran CV, Thiyagarajan P, Deepak HB, Agarwal A.
R&D centre, Natural Remedies Pvt. Ltd., Bangalore, India.
The aim of the current study is to probe the anti-inflammatory/anti-allergic potential of seven phytoconstituents (andrographolide, neoandrographolide, isoandrographolide, andrograpanin, 14-deoxy-11,12-didehydroandrographolide, 7-O-methylwogonin and skullcapflavone-I) isolated from Andrographis paniculata (King of bitters) on the production of key inflammatory/allergic mediators (NO, PGE(2), IL-1 beta, IL-6, LTB(4), TXB(2) and histamine). The results demonstrated that andrographolide, isoandrographolide, 7-O-methylwogonin and skullcapflavone-I significantly inhibited LPS stimulated NO and PGE(2) release in J774A.1 macrophages. Andrographolide, isoandrographolide and 7-O-methylwogonin showed considerable inhibition of IL-1 beta production in LPS elicited macrophages. LPS induced IL-6 production was significantly inhibited by andrographolide, isoandrographolide and skullcapflavone-I in a concentration dependent manner. The results revealed that andrographolide, isoandrographolide and skullcapflavone-I significantly decreased TXB(2) release in A23187 activated HL-60 promyelocytic cells. Furthermore, the anti-allergic properties of the phytoconstituents was investigated on A23187 induced LTB(4) production (HL-60 cells) and histamine release (RBL-2H3 basophilic cells). The results showed that only skullcapflavone-I and 7-O-methylwogonin showed marked inhibitory effect on LTB(4) production, however, only 7-O-methylwogonin exerted dose-dependent inhibition towards histamine release. Therefore, this study indicates that some of these phytoconstituents exhibit potent anti-inflammatory/anti-allergic effects by modulating different inflammatory/allergic mediators. Hence, these phytoconstituents might provide useful phytomedical treatment against variety of inflammatory and allergic disorders.
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