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Mesenchymal stem cells (MSCs) are bone marrow-derived, self-renewing and multipotent progenitors. MSCs have been shown to be capable of differentiating into multiple cell types including adipocytes, chondrocytes, osteocytes, and cardiomyocytes.
R&D Systems offers kits for the maintenance and expansion of MSCs, as well as kits and reagents designed to promote and identify the progression of MSCs into osteogenic,adipogenic, and chondrogenic lineages.
Media & Supplements for Mesenchymal Stem Cell Expansion & Differentiation
StemXVivo MSC Expansion Media is a complete media for the expansion of mesenchymal stem cells (MSCs). All the components have been selected and optimized for culturing human and mouse MSCs. R&D Systems also offers base media and supplements specially formulated to direct MSC differentiation into adipogenic, chondrogenic, and osteogenic lineages.
Please see pages 20-21, 22-23, and 24-25 for protocols using StemXVivo Media and supplements for the differentiation of MSCs into osteogenic, adipogenic, and chondrogenic lineages, respectively. A protocol describing the expansion of MSCs can be found at www.RnDSystems.com/go/MSCExpansionProtocol.
Phenotypic Analysis of Human MSCs by Flow Cytometry. MSCs were expanded using StemXVivo MSC Expansion Media (Catalog # CCM004). Green histograms indicate cells stained with markers of undifferentiated MSCs including, anti-CD105 (Catalog # FAB10971P) or CD90 (Catalog # FAB2067P). The open histograms show isotype-matched control staining. MSCs lack expression of CD34.
Mesenchymal Stem Cell Functional Identification Kits
Mesenchymal Stem Cell Functional Identification Kits contain specially formulated Adipogenesis, Chondrogenesis, and Osteogenesis Media Supplements that can be used to effectively differentiate MSCs or other candidate cell populations, into adipogenic, chondrogenic, or osteogenic lineages. A panel of antibodies are included for identification of the mature phenotypes of each lineage.
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