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- 请问,为什么BSP只能扩增300-500bp产物
- 请问我想研究两个具有不同的SNP甲基化酶对基因组修饰 ...
- 无法
- 发放范围
- 很好的学习材料
- 很受用
- MeDIP-Seq能发现有差异的具体哪个基因吗?
- HRM的引物怎么设计啊
- 你好 我只想看一下甲基化水平在各组间有没有差异 做MSP可以 ...
- 收费怎么样?
- 您好,我想要咨询一下,BSP绘制了圆点图后最终的甲基化 ...
- 你好,请问测序reads在不同基因功能元件上的分布为 ...
- diffscoredelta beta 这两个是如何来的,它 ...
- 焦磷酸测序一般一个反应多少钱啊
- 看不懂
- MSP后的pcr,目的条带很暗,而且周围很模糊,什么原 ...
- 如果只是想针对某几个特定基因做甲基化,选择哪种或几种 ...
- 蛮好的,谢谢
- i learned. it is useful. i am going to undert ...
- diffscore怎么计算的
- 谢谢分享!
- 挺好的学习材料!
- 还不错
- 非常不错!谢谢!
高通量甲基化研究技术-芯片和二代测序
Schematic outline of MeDIP. Genomic DNA is sheared into ~400-700bp using sonication and subsequently denatured. Incubation in 5mC antibodies, along with standard immunoprecipitation, enriches for fragments that are methylated (IP fraction). This IP fraction can become the input sample to one of two DNA detection methods: array hybridization using high-density microarrays (A) or high-throughput sequencing using the latest in sequencing technology (B). Output from these methods are then analyzed for methylation patterns to answer the biological question.
编辑: gaowei2010