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高通量甲基化研究技术-芯片和二代测序

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发布日期:2012-09-20 21:08 文章来源:丁香园
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关键词: 甲基化 技术专题 欧易生物 丁香通 丁香园   点击次数:

Schematic outline of MeDIP. Genomic DNA is sheared into ~400-700bp using sonication and subsequently denatured. Incubation in 5mC antibodies, along with standard immunoprecipitation, enriches for fragments that are methylated (IP fraction). This IP fraction can become the input sample to one of two DNA detection methods: array hybridization using high-density microarrays (A) or high-throughput sequencing using the latest in sequencing technology (B). Output from these methods are then analyzed for methylation patterns to answer the biological question.

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