你知道吗? 详细 >>
内参
内参在生物学研究中非常常见。例如RT-PCR或western blot实验中常用表达量稳定的管家基因/蛋白作为评估不同样本之间目标基因或蛋白变化的参考。以western blot为例,如果加药处理前后GAPDH或tublin、actin等基本没有变化,而目标蛋白表达变化明显,则说明“加药”过程中目标蛋白的表达受到影响。内参的引入可以排除上样量不同或样本处理过程中的偏差导致的可能误差···
经典文献 更多 >>
- Conserved seed pairing often flanked by ad ...
- MicroRNA expression profiles classify ...
- Prediction of mammalian microRNA targe ...
- A microRNA expression signature of human s ...
- Combinatorial microRNA target predic ...
- The nuclear RNase III Drosha initiates mic ...
- A microRNA polycistron as a potential human ...
- RAS is regulated by the let-7 MicroRNA family
- MicroRNA signatures in human cancers
- Human microRNA genes are frequently loca ...
microRNA定量茎环法
编辑: helen